• 06/1991 BS Life Sciences
  Punjabi University, Patiala, India.

• 07/1993 MS Biotechnology
  Department of Biotechnology, Punjabi University, Patiala, India.

• 09/1998 Ph.D. Biotechnology
  "Biochemical Studies in Growing Bamboo, Dendrocalamus strictus"
  School of Biotechnology, Thapar Institute of Engineering and Technology, India .

• Postdoctoral Research Associate,
  Dept. of Biochemistry & Molecular Biology, University of Medicine and Dentistry New Jersey, Newark.

• Postdoctoral Research Associate,
  Dept. of Microbiology & Immunology, Weill Medical College, New York.

The long-term research goal of the lab is to apply computer modeling to gain insight into cellular signal transduction pathways, specifically to provide deeper insight into both the normal and aberrant subcellular targeting of domains contained in proteins which are part of macromolecular complexes and function in various biological processes.

The current focus is on understanding the molecular basis of interactions of specialized protein domains like the Pleckstrin Homology (PH) domain with lipids and protein partners that contribute to their subcellular localization and function. The protein/membrane and protein/protein complexes that function in signaling pathways are often not amenable to traditional structure determination.

The integration of bioinformatics tools to analyze genomic data with detailed computer calculations of the physical interactions can provide novel insights into the molecular basis of the formation and regulation of these complexes and, thus, allow the suggestion of rational and experimentally testable predictions as to their function. This computational analysis can be successfully extended to a genome-wide level, allowing for the analysis of emerging families of specialized protein domains with multiple roles at the whole genome level.

• Singh, S. and Murray, D. (2003). Molecular modeling of the membrane targeting of Phospholipase C Pleckstrin homology domains. Protein Sci.12, 1934-1953.

• Yu, J.W., Mendrola, J.M., Audhya, A., Singh, S., Keleti, D., DeWald, D.B., Murray, D., Emr, S.D., and Lemmon, M.A. (2004). Genome-Wide Analysis of Membrane Targeting by S. cerevisiae Pleckstrin Homology (PH) Domains. Molecular Cell. 13, 677-688.

• Stahelin, R.V., Ananthanarayanan, B., Blatner, N.R., Singh, S., Bruzik, K.S., Murray, D., and Cho, W. (2004). Mechanism of membrane binding of the phospholipase D1 PX domain. J Biol Chem. 279, 54819-54826

• Hisert, K.B., MacCoss, M., Shiloh, M.U., Darwin, K.H., Singh, S., Jones, R.A., Ehrt, S., Zhang, Z., Gaffney, B.L., Gandotra, S., Holden, D.W., Murray, D. and Nathan, C. (2005). An EAL domain protein of Salmonella controls bacterial survival in mice, anti-oxidant defense, and killing of macrophages: role of cyclic-diGMP. Molecular Microbiology. 56, 1234-1245.

• Mulgrew-Nesbitt, A., Diraviyam, K., Wang, J., Singh, S., Murray, P., Li, Z., Rogers, L., Mirkovic, N. and Murray, D. (2006). The role of electrostatics in protein-membrane interactions. Lipid-protein Interactions. Biochim. Biophys. Acta. 176,1812-826.

Meeting Presentations

• Singh S.M., Gupta V., and Wagner B.J. Mouse lens gene expression of proteasome homologous housekeeping and inducible subunits. XIV ICER Meeting, Santa Fe, New Mexico (October, 2000). Wagner, B.J, Singh, S. M., Awasthi, N., Gupta, V. and Egwuagu, C.E. Evidence for non-immune function for immunoproteasome in lens. Fourth International Workshop on Proteasomes, Clermont-Ferrand, France (April 2001).
• Singh, S. M. and Murray, D. Molecular modeling studies of Phospholipase C PH domains. Cornell Keck Program in Cellular and Molecular Biophysics of Signal Transduction, South Fallsburg, New York (June, 2001).
• Singh, S.M. and Murray, D. Molecular modeling studies of Phospholipase C PH domains. Biophysical J. (Annual Meeting Abstracts) 2002, 820-Pos.
• Singh, S.M. and Murray, D. Molecular modeling studies of Phospholipase C PH domains. 42nd Annual Meeting, The American Society for Cell Biology, San Francisco, California (December 2002)
• Singh, S. M., Li, Z.D. and Murray, D. Membrane targeting of Phospholipase C C2 Domains. Biophysical J. (Annual Meeting Abstracts) 2003, 270-Pos.
• Singh, S.M. and Murray, D. Role of non-specific electrostatics in the membrane targeting of pleckstrin homology domains. Biophysical J. (Annual Meeting Abstracts) 2004, 2906-Pos. Newell, S.A., Polle, J. and Singh, S. Molecular Modeling Studies of Chlamydomonas reinhardtii LHC Proteins. 45th Annual Meeting, The American Society for Cell Biology, San Francisco, California (December 2005).
• Wywial, E., and Singh, S. Molecular Modeling Studies of the Pleckstrin Homology domains of the Gab family of proteins. 46th Annual Meeting, The American Society for Cell Biology, San Diego, California (December 2006).

Note: Maiden name: Shaneen Jolly

Stacey Garil Womack award for cancer research, Stacey Garil Womack Memorial Fund, $15,000, 1year (2004-2005)

PSC-CUNY, The Research Foundation, $4040, 1 year (2005-2006)

PSC-CUNY, The Research Foundation, $3500, 1 year (2006-2007) Arabidopsis 2010, NSF, $ 740,100, 4 years (2006-2010)