Laura J. Juszczak
Assistant Professor
Chemistry
Location: 3119 Ingersoll Hall
Phone: 718.951.5000 x1426
Fax: 718.951.4607
Email:
Laura J. Juszczak's Web site
After several years as a painting conservator, Laura Juszczak returned to New York University with the aim of becoming an art conservation scientist. On the way to a master's degree, however, her focus changed from the study of inorganic to organic pigments. She stayed on to complete a Ph.D.; her dissertation focused on the energy transfer mechanism in light-harvesting blue-green algae. Continuing with spectroscopy, she worked as a postdoc in the laser lab of Professor Joel M. Friedman, primarily studying hemoglobin mutants using the laser technique of UV resonance Raman(UVRR) spectroscopy. She stayed on at the Albert Einstein College of Medicine as a faculty associate. With her faculty appointment in 2006 at Brooklyn College, Juszczak earned NIH funding to apply UVRR and other spectroscopic techniques, together with molecular modeling simulations, to study the reporter molecule and amino acid, tryptophan, in proteins.
Education:
Postdoctoral studies, Albert Einstein College of Medicine - 1999 (Laser Spectroscopy of Proteins)
Ph.D., New York University - 1993 (Chemistry, Physical)
M.S., New York University - 1986 (Chemistry)
M.S., University of Delaware - 1979 (Art Conservation)
A.B., Wellesley College - 1976 (Art History)
Areas of Expertise:
Laura Juszczak's area of expertise is biophysical chemistry; her tools are spectroscopic, primarily UV resonance Raman. This technique is used to selectively probe aromatic residues in proteins, primarily tryptophan but also tyrosine. Such studies reveal details about protein conformation in both the normal and diseased state. This information allows us to understand the molecular level detail of the disease process, and helps researchers to design effective drugs.
Doctoral Rotation Projects:
Laura Juszczak's interest in tryptophan and indole chemistry has resulted in the following project that students may chose in their first-year rotation. The project involves the steady state and time-resolved spectroscopic examination of indole derivatives and tryptophan dipeptides along with molecular dynamics simulation of dipeptide conformers to arrive at a set of spectroscopic markers for identifying residues surrounding tryptophan in a protein matrix.
Books and Publications
Eisenberg, Azaria Solomon and Laura J. Juszczak. "Relating Trp-Glu Dipeptide Fluorescence to Molecular Conformation: The Role of the Discrete chi 1 and chi 2 Angles." Journal of Computational Chemistry. Article first published online, April 8 | DOI: 10.1002/jcc.23288. (Books and Publications: Peer Reviewed Article) 2013
Eisenberg, A.S. and Laura Juszczak. "Correlation of TrpGly and GlyTrp Rotamer Structure with W7
and W10 UV Resonance Raman Modes and Fluorescence Emission Shifts" Journal of Amino Acids. DOI: 10.1155/2012/735076. (Books and Publications: Forthcoming Publications) 2012
Meng, Xianwei, Trisheena Harricharran and Laura Juszczak. "A Spectroscopic Survey of Substituted Indoles Reveals Consequences of a Stabilized 1Lb Transition." Photochemistry and Photobiology. DOI 10.1111/j1751-1097. (Books and Publications: Forthcoming Publications) 2012
Juszczak, L.J. and R.Z.B. Desamero. "Extension of the Tryptophan Dihedral Angle - W3 Band Frequency Relationship to a Full Rotation: Correlations and Caveats." Biochemistry 48: 2777-87. (Books and Publications: Peer Reviewed Article) 2009
Maliga, Z., J. Xing, H. Cheung, L.J. Juszczak, J.M. Friedman and S.S. Rosenfeld. "A Pathway of Structural Changes Produced by Monastrol Binding to Eg5." Journal of Biological Chemistry 281: 7977-82. (Books and Publications: Peer Reviewed Article) 2006
Samuni, U., C.J. Roche, D. Dantsker, L.J. Juszczak and J. M. Friedman. "Modulation of Reactivity and Conformation within the T-quaternary State of Human Hemoglobin: The Combined Use of Mutagenesis and Sol-Gel Encapsulation." Biochemistry 45: 2820-35. (Books and Publications: Peer Reviewed Article) 2006
Juszczak, L., U. Samuni and J. M. Friedman. "The Conformational and Functional Significance of the α140 Side Chain in HbA: A UV and Visible Resonance Raman Study of the Three α140 Mutants." Journal of Raman Spectroscopy 36: 350-58. (Books and Publications: Peer Reviewed Article) 2005
Kavanaugh, J.S., L.J. Juszczak, P.H. Rogers, A. Arnone, Hilda L. Hui, Anita Wierzba, Alice DeYoung, Laura D. Kwiatkowski, Robert W. Noble, Eric S. Peterson and Joel M. Friedman. "Intersubunit Interactions Associated with Tyr42 alpha Stabilize the Quaternary-T Tetramer but Are Not Major Quaternary Constraints in Deoxyhemoglobin." Biochemistry 44: 3806-20. (Books and Publications: Peer Reviewed Article) 2005
"Comparative Vibrational Spectroscopy of Intracellular Tau and Extracellular Collagen I Reveals Parallels of Gelation and Fibrillar Structure." Journal of Biological Chemistry 279: 7395-404 and 18098. (Books and Publications: Peer Reviewed Article) 2004
Awards, Honors and Fellowships
Lipke, P., L. Quadri, L. Juszczak and T. Muth. MRI-R2 joint proposal for major research instrument: "Acquisition of a Plasmon Resonance Spectrometer: Metal Surface Affinity and Kinetics for Irreversible-Binding Proteins as Measured by SPR Spectroscopy." Funded August 2010-July 31, 2013. (Grants and Fellowships) 2010
NIH SC02 proposal 1SC2GM092291-01, for "Spectroscopic Markers for Blue-Fluorescing Tryptophan in Proteins." Funded June 2010-May 2013. (Grants and Fellowships) 2010
PSC-CUNY Award #62490-00-40, for "A Small Drug-Like Molecule Inhibits Amyloid Beta 42 Oligomerization and Toxicity." (Grants and Fellowships) 2009
PSC-CUNY Award #60087-37-38, for "Two-Dimensional Spectroscopic Analysis of Protein Unfolding/Refolding by FTIR Spectroscopy." (Grants and Fellowships) 2007
Conferences, Seminars and Symposiums
Eisenberg, A., M. Nathan and L.J. Juszczak. " Backbone Stretched; Backbone Curled: Conformational Similarity for Trp Dipeptides Parallels Fluorescence Emission and UVRR W10 Shifts." 56th International Biophysical Society Annual Meeting. San Diego, Feb. 25-29. (Conferences, Seminars and Symposiums: Conference Presentation) 2012
"Ultraviolet Resonance Raman Spectroscopy: Versatility at a Single Wavelength." CUNY Graduate Center. BICM. March 15. (Conferences, Seminars and Symposiums: Invited Talk) 2011
Eisenberg, Azaria and L.J. Juszczak. "Tracing the Fluorescence Shift of Tryptophan Dipeptides" poster. International Biophysical Society Meeting. Baltimore, March 2-4. (Conferences, Seminars and Symposiums: Conference Presentation) 2011
Meng, X., A. Eisenberg, T. Harricharan and L. Juszczak. "Electron Density of Indole Derivatives, Calculation and Experiment," Marie Curie Nobel Centennial Symposium. Baruch College (CUNY). New York, Nov. 14. (Conferences, Seminars and Symposiums: Conference Presentation) 2011
Marin, Tania and Laura Juszczak. "A Study of Unfolding and the Beta Sheet to Alpha Helix Conformational Switch in Beta Lactoglobulin" poster. 54th International Biophysical Society Annual Meeting. San Francisco, Feb. 20-24. (Conferences, Seminars and Symposiums: Conference Presentation) 2010
Juszczak, L. and R. Desamero. "Extension of the Chi(2,1) Dihedral Angle - W3 Band Frequency Relationship to a Full Rotation: Correlations and Caveats" poster. 53rd International Biophysical Society Meeting. Boston, Feb. 28-March 4. (Conferences, Seminars and Symposiums: Conference Presentation) 2009
Professional Leadership
Undergraduate science fair judge, NYC Science and Engineering Fair. City College of New York (CUNY). New York, March 4. (Professional Leadership: Public Service) 2012
Arrangements co-coordinator, 25th Annual H. Martin Friedman Lecture, Brooklyn College, October. (Professional Leadership: Organizational Leadership Position) 2009
Faculty Membership Committee, Biochemistry Department, CUNY Graduate Center. (Professional Leadership: Committee Service) 2008
Member, Graduate Curriculum Committee, Brooklyn College Faculty Council. (Professional Leadership: Committee Service) 2008
Pedagogical Achievements
Lecturer, BICM 88800, "Protein Adsorption to Surfaces." Biochemistry Department, CUNY Graduate Center. 2010
Undergraduate mentor for Barnard College student Viktoriya Rutkovskaya, on "Fluorescence Excitatation and Emission Study of Indole in Graduated Solutions of N-pentane and N-butanol." June-July. 2010
Visiting master's student mentor for Fenneke KleinJan of Gronningen University, the Netherlands, on master's thesis chapter, "UV Resonance Raman Analysis of Proteins With Highly Blue-shifted Tryptophan Fluorescence." November 2009-May 2010. 2010
Visiting scientist collaboration. Sarah Paquette, Ph.D., professor, College of the Menominee Nation. Keshena, Wis. Development of a program for two-dimensional correlation analysis using Mathematica software. June-July. 2010
Community Activities
Brooklyn College/CUNY representative, NIH Graduate & Professional School Fair, Natcher Conference Center, National Institutes of Health, Bethesda, Md., July 8. 2010
Other Professional Activities
"Spectroscopic Study of Blue-shifted Tryptophan Fluorescence in Proteins" seminar and outreach. College of the Menominee Nation. Keshena, Wis., Oct. 26. 2010
"Spectroscopic Study of Blue-shifted Tryptophan Fluorescence in Proteins" seminar. Department of Chemistry, Illinois Institute of Technology. Chicago, Oct. 25. 2010
MBRS - Support of Competitive Research SCORE Program meeting. National Institutes of Health. Bethesda, Md., Dec. 2-3. 2010
PSC-CUNY Panel on Grant Awards in Chemistry. 2010
Attended "Drug Discovery for Neurodegeneration." Alzheimer's Drug Discovery Foundation Conference. Washington, D.C., Feb. 4-5. 2008
U.S. Provisional Patent Application no. 60/802,972, for "Methods of Dissolving beta-Sheet Proteins and Uses Thereof." 2006





