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Richard Magliozzo
Professor
Chemistry
Location: 3148 Ingersoll Hall
Phone: 718.951.5000 x2845
Fax: 718.951.4607
Email:
Richard Magliozzo recieved his bachelor's degree in chemistry from Hunter College. He did his postdoctoral training at Columbia University, Department of Biochemistry, Albert Einstein College of Medicine, Department of Pharmacology and Department of Physiology and Biophysics. He is the Levy-Kosminsky Professor in Physical Chemistry (2004-10).
Education:
Ph.D., Graduate School & University Center - 1981 (Biochemistry)
Areas of Expertise:
Magliozzo teaches biochemistry, physical biochemistry and enzymology. His expertise lies in physical biochemistry of metalloproteins and metalloenzymes, electron paramagnetic resonance spectroscopy, rapid reaction kinetics (optical stopped-flow and rapid freeze-quench methods.
Doctoral Rotation Projects:
Biochemistry/Chemistry Ph. D. Program mentor (subdisciplines physical, inorganic, biophysical). Magliozzo's research involves defining structure/function of metalloenzymes using spectroscopy and kinetics measurements, focused on catalase-peroxidase (KatG) a heme enzyme from M. tuberculosis. Antibiotic resistance in TB-infected people arises from mutations in the gene for the KatG enzyme. Wild-type and drug resistance mutant KatG enzymes are being studied. His lab group comprises postdocs, chemistry/biochemistry Ph.D. and M.A. students, undergraduates and high school students, and is funded by the National Institute of Allergy and Infectious Diseases/NIH, NSF, CUNY. Collaborations: resonance Raman, crystallography, theoretical chemistry labs. In lab rotations, students learn bacterial culture, molecular biology, protein purification, HPLC, rapid kinetics, EPR in biology, radical enzyme reactions, ITC, enzymology, drug chemistry and chemical calculations.
Books and Publications
Zhang, L., E.M. Andersen, A. Khajo, R.S. Magliozzo and R.L. Koder. "Dynamic Factors Affecting Gaseous Ligand Binding in an Artificial Oxygen Transport Protein." Biochemistry 52: 447-55. doi: 10.1021/bi301066z. (Books and Publications: Peer Reviewed Article) 2013
Carreira, M., R. Calvo-Sanjuán, M. Sanaú, X. Zhao, R.S. Magliozzo, I. Marzo and M. Contel. "Cytotoxic Hydrophilic Iminophosphorane Coordination Compounds of d8 Metals. Studies of their Interactions with DNA and HSA." Journal of Inorganic Biochemistry 116: 204-14. PMID:23063789. (Books and Publications: Peer Reviewed Article) 2012
Ranguelova, K., A.B. Rice, A. Khajo, M. Triquigneaux, S. Garantziotis, R.S. Magliozzo and R.P. Mason. "Formation of Reactive Sulfite-derived Free Radicals by the Activation of Human Neutrophils: An ESR Study." Free Radical Biology & Medicine 52.8: 1264-71. Epub Feb. 2. PMID: 22326772. (Books and Publications: Peer Reviewed Article) 2012
Zhang, Qi, Abdelahad Khajo, Tsunehiro Sai, Ian de Albuquerque, Richard Magliozzo and Kalle Levon. "Intramolecular transport of charge carriers in trimeric aniline upon a three-step acid doping process." Journal of Physical Chemistry A 116: 7629-35. PMID:22784005. (Books and Publications: Peer Reviewed Article) 2012
Zhao, X., A. Khajo, S. Jarrett, J. Suarez, Y. Levitsky, R.M. Burger, A.A. Jarzecki and R.S. Magliozzo. "Specific Function of the Met-Tyr-Trp Adduct Radical and Residues Arg-418 and Asp-137 in the Atypical Catalase Reaction of Catalase-Peroxidase KatG." Journal of Biological Chemistry 287: 37057-65. PMID: 22918833. (Books and Publications: Peer Reviewed Article) 2012
Khajo, A., R.A. Bryan, M. Friedman, R.M. Burger, Y. Levitsky, A. Casadevall, R.S. Magliozzo and E. Dadachova. "Protection of Melanized Cryptococcus Neoformans From Lethal Dose Gamma Irradiation Involves Changes in Melanin's Chemical Structure and Paramagnetism." PLoS One 6.9: e25092. (Books and Publications: Peer Reviewed Article) 2011
Martínez, A., J. Suárez, T. Shand, R.S. Magliozzo and R.A. Sánchez-Delgado. "Interactions of arene-Ru(II)-chloroquine Complexes of Known Antimalarial and Antitumor Activity With Human Serum Aalbumin (HSA) and Transferrin." Journal of Inorganic Biochemistry 105: 39-45. PMID: 21113330. (Books and Publications: Peer Reviewed Article) 2011
Hirota, S., N. Tanaka, I. Micetic, P. Di Muro, S. Nagao, H. Kitagishi, K. Kano, R.S. Magliozzo, J. Peisach, M. Beltramini and L. Bubacco. "Structural Basis of the Lactate-dependent Allosteric Regulation of Oxygen Binding in Arthropod Hemocyanin." Journal of Biological Chemistry April 20. [Epub ahead of print.] PMID: 20406810. (Books and Publications: Peer Reviewed Article) 2010
Zhao, X., J. Suarez, A. Khajo, S. Yu, L. Metlitsky and R.S. Magliozzo. "A Radical on the Met-Tyr-Trp Modification Required for Catalase Activity in Catalase-peroxidase Is Established by Isotopic Labeling and Site-directed Mutagenesis." Journal of the American Chemical Society 132: 8268-69. (Books and Publications: Peer Reviewed Article) 2010
Colangeli, R., A. Haq, V.L. Arcus, E. Summers, R.S. Magliozzo, A. McBride, A.K. Mitra, M. Radjainia, A. Khajo, W.R. Jacobs Jr., P. Salgame and D. Alland. "The Multifunctional Histone-like Protein Lsr2 Protects Mycobacteria Against Reactive Oxygen Intermediates." Proceeding of the National Academy of Sciences USA 106.11, March 17: 4414-18. Epub 2009 Feb. 23. PMID: 19237572. (Books and Publications: Peer Reviewed Article) 2009
Suarez, J., K. Ranguelova, A.A. Jarzecki, J. Manzerova, V. Krymov, X. Zhao, S. Yu, L. Metlitsky, G.J. Gerfen and R.S. Magliozzo. "An Oxyferrous Heme/protein-based Radical Intermediate Is Catalytically Competent in the Catalase Reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG)." Journal of Biological Chemistry 284.11, March 13 :7017-29. Epub 2009 Jan. 12. PMID: 19139099. (Books and Publications: Peer Reviewed Article) 2009
Suarez, J., K. Ranguelova, J.P. Schelvis and R.S. Magliozzo. "Antibiotic Resistance in Mycobacterium Tuberculosis: Peroxidase Intermediate Bypass Causes Poor Isoniazid Activation by the S315G Mutant of M. tuberculosis catalase-peroxidase (KatG)." Journal of Biological Chemistry 284.24, June 12: 16146-55. Epub 2009 April 9. PMID: 19363028. (Books and Publications: Peer Reviewed Article) 2009
Zhao, X., S. Yu, K. Ranguelova, J. Suarez, L. Metlitsky, J.P. Schelvis and R.S. Magliozzo. "Role of the Oxyferrous Heme Intermediate and Distal Side Adduct Radical in the Catalase Activity of Mycobacterium tuberculosis KatG Revealed by the W107F Mutant." Journal of Biological Chemistry 284.11, March 13: 7030-37. (Books and Publications: Peer Reviewed Article) 2009
Hirota, S., T. Kawahara, M. Beltramini, P. Di Muro, R.S. Magliozzo, J. Peisach, L.S. Powers, N. Tanaka, S. Nagao and L. Bubacco. "Molecular Basis of the Bohr Effect in Arthropod Hemocyanin." Journal of Biological Chemistry 283.46, Nov. 14: 31941-48. Epub 2008 Aug. 25. (Books and Publications: Peer Reviewed Article) 2008
Ranguelova, K., J. Suarez, L. Metlitsky, S. Yu, S.Z. Brejt, S.Z. Brejt, L. Zhao, J.P. Schelvis and R.S. Magliozzo. "Impact of Distal Side Water and Residue 315 on Ligand Binding to Ferric Mycobacterium tuberculosis catalase-peroxidase (KatG)." Biochemistry 47.47, Nov. 25: 12583-92. PMID: 18956888. (Books and Publications: Peer Reviewed Article) 2008
Ranguelova, K., J. Suarez, R.S. Magliozzo and R.P. Mason. "Spin Trapping Investigation of Peroxide- and Isoniazid-induced Radicals in Mycobacterium tuberculosis catalase-peroxidase." Biochemistry 47.43, Oct. 28: 11377-85. Epub 2008 Oct 2. PMID: 18831539. (Books and Publications: Peer Reviewed Article) 2008
Kapetanaki, S.M., X. Zhao, S. Yu, R.S. Magliozzo and J.P.M. Schelvis. "Modification of the Active Site of Mycobacterium tuberculosis KatG After Disruption of the Met-Tyr-Trp Cross-linked Adduct." Journal of Inorganic Biochemistry 101.3: 422-33. (Books and Publications: Peer Reviewed Article) 2007
Ouellet, H, K. Ranguelova, M. Labarre, J.B. Wittenberg, B.A. Wittenberg, R.S. Magliozzo and M. Guertin. "Reaction of Mycobacterium tuberculosis Truncated Hemoglobin O With Gydrogen Peroxide: Evidence for Peroxidatic Activity and Formation of Protein-based Radicals." Journal of Biological Chemistry 282: 7491-503. (Books and Publications: Peer Reviewed Article) 2007
Ranguelova, Kalina, Stefania Girotto, Gary J. Gerfen, Shengwei Yu, Javier Suarez, Leonid Metlitsky and Richard S. Magliozzo. "Radical Sites in Mycobacterium tuberculosis KatG Identified Using Electron Paramagnetic Resonance Spectroscopy, the Three-dimensional Crystal Structure, and Electron Transfer Couplings." Journal of Biological Chemistry 282: 6255-64. (Books and Publications: Peer Reviewed Article) 2007
Zhao, X., S. Yu and R.S. Magliozzo. "Characterization of the Binding of Isoniazid and Analogues to Mycobacterium tuberculosis catalase-peroxidase." Biochemistry 46: 3161-70. (Books and Publications: Peer Reviewed Article) 2007
Zhao, X., H. Yu, S. Yu, F. Wang, J.C. Sacchettini and R.S. Magliozzo. "Hydrogen Peroxide-Mediated Isoniazid Activation Catalyzed by Mycobacterium tuberculosis catalase-peroxidase (KatG) and Its S315T Mutant." Biochemistry 45: 4131-40. (Books and Publications: Peer Reviewed Article) 2006
Kapetanaki, S.M., S. Chouchane, S. Yu, R.S. Magliozzo and J.P. Schelvis. "Resonance Raman Spectroscopy of Compound II and Its Decay in Mycobacterium tuberculosis catalase-peroxidase KatG and Its Isoniazid Resistant Mutant S315T." Journal of Inorganic Biochemistry 99: 1401-06. (Books and Publications: Peer Reviewed Article) 2005
Kapetanaki, S.M., S. Chouchane, S. Yu, X. Zhao, R.S. Magliozzo and J.P. Schelvis. "Mycobacterium tuberculosis KatG(S315T) catalase-peroxidase Retains All Active Site Properties for Proper Catalytic Function." Biochemistry 44: 243-52. (Books and Publications: Peer Reviewed Article) 2005
Kapetanaki, S.M., S. Chouchane, S. Yu, X. Zhao, R.S. Magliozzo and J.P. Schelvis. "Mycobacterium tuberculosis KatG(S315T) catalase-peroxidase Retains All Active Site Properties for Proper Catalytic Function." Biochemistry 44: 243-52. (Books and Publications: Peer Reviewed Article) 2005
Zhao, X., H. Yu, S. Yu, F. Wang, J.C. Sacchettini and R.S. Magliozzo. "Hydrogen Peroxide-mediated Isoniazid Activation Catalyzed by Mycobacterium tuberculosis catalase-peroxidase (KatG) and Its S315T Mutant." Biochemistry 45: 4131-40. (Books and Publications: Peer Reviewed Article) 2005
Zhao, X., S. Girotto, S. Yu and R.S. Magliozzo. "Evidence for Radical Formation at Tyr-353 in Mycobacterium tuberculosis catalase-peroxidase (KatG)." Journal of Biological Chemistry 279: 7606-12. (Books and Publications: Peer Reviewed Article) 2004
Chouchane, S., S. Girotto, S. Kapetanaki, J.P.M. Schelvis and R.S. Magliozzo. "Analysis of Heme Structural Heterogeneity in Mycobacterium tuberculosis catalase-peroxidase (KatG)." Journal of Bioligcal Chemistry 278: 8154-62. (Books and Publications: Peer Reviewed Article) 2003
Kapetanaki, S., S. Chouchane, S. Girotto, S. Yu, R.S. Magliozzo and J.P. Schelvis. "Conformational Differences in Mycobacterium tuberculosis catalase-Peroxidase KatG and Its S315T Mutant Revealed by Resonance Raman Spectroscopy." Biochemistry 42: 3835-45. (Books and Publications: Peer Reviewed Article) 2003
Kapetanaki, S., S. Chouchane, S. Girotto, S. Yu, R.S. Magliozzo and J.P.M. Schelvis. "Conformational Differences in Mycobacterium tuberculosis catalase-peroxidase KatG and Its S315T Mutant Revealed by Resonance Raman Spectroscopy." Biochemistry 42: 3835-45. (Books and Publications: Peer Reviewed Article) 2003
Yu, S., S. Girotto, C. Lee and R.S. Magliozzo. "Reduced Affinity for Isoniazid in the S315T Mutant of Mycobacterium tuberculosis KatG Is a Key Factor in Antibiotic Resistance." Journal of Biological Chemistry 278: 14769-75. (Books and Publications: Peer Reviewed Article) 2003
Yu, S., S. Girotto, X. Zhao and R.S. Magliozzo. "Rapid Formation of Compound II and a Tyrosyl Radical in the Y229F Mutant of Mycobacterium tuberculosis catalase-peroxidase Disrupts Catalase but Not Peroxidase Function. Journal of Biological Chemistry 278: 44121-27. (Books and Publications: Peer Reviewed Article) 2003
Awards, Honors and Fellowships
NSF Award, year 3. Award No. CHE-1058116. $140,000. (Grants and Fellowships) 2013
2R56AI060014-06A1 NIH Bridge Award, for "Catalysis of Isoniazid Action by M. tuberculosis KatG." $509,394; Sept. 23. (Grants and Fellowships) 2010
Levy-Kosminsky Professorship in Physical Chemistry. (Awards and Honors) 2010
Levy-Kosminsky Professorship in Physical Chemistry. (Awards and Honors) 2009
Levy-Kosminsky Professorship in Physical Chemistry. (Awards and Honors) 2008
Levy-Kosminsky Professorship in Physical Chemistry. (Awards and Honors) 2006
National Institute of Allergy and Infectious Diseases, National Institutes of Health, for "Catalysis of Isoniazid Activation by M. tuberculosis catalase-peroxidase (KatG)." $1,767,000; Feb. 1, 2005-Jan. 31, 2010. (Grants and Fellowships) 2005
Levy-Kosminsky Professor in Physical Chemistry. (Awards and Honors) 2004
PSC-CUNY grant, for "Mechanism of Active-site Adduct Formation in M. tuberculosis KatG." July 2004-June 30, 2006. (Grants and Fellowships) 2004
CUNY Instrumentation Program Award, for "Acquisition of a MicroCal Isothermal Titration Calorimeter for Education and Research." $48,000; July. (Grants and Fellowships) 2002
NSF/Biological Sciences Directorate MRI Award, for "Acquisition of an Electron Paramagnetic Resonance Spectrometer for Use in Research, Undergraduate and Graduate Curricula at Brooklyn College." $181,180; July 1. (Grants and Fellowships) 2002
National Institute of Allergy and Infectious Diseases, National Institutes of Health, for "Catalase-peroxidase Catalysis and Antibiotic Activation." $930,767; Jan. 1, 1999-May 31, 2003. (Grants and Fellowships) 1999
Conferences, Seminars and Symposiums
"Function of the Met-Tyr-Trp cofactor in catalase-peroxidase." Gordon Research Conferences: Protein Cofactors, Radicals and Quinones. Mount Holyoke, Mass., July. (Conferences, Seminars and Symposiums: Invited Talk) 2012
Community Activities
Editorial board, Journal of Biological Chemistry. 2011
Other Professional Activities
Mentoring: postdoctoral fellows (past): Dr. Kalina Ranguelova (currently a research scientist at the NIH/NIEHS), Dr. Salem Chouchane (currently a research fellow at Philip Morris USA) and Dr. Wenhai Zhang (currently a research scientist at Glaxo-Smith-Kline); graduate students (current): Javier Suarez and Prachi Kulkarni (both Ph.D. Program in Biochemistry), Leonid Metlitsky (M.A. student) and Abdelahad Khajo; graduate students (past): Stefania Girotto (Ph.D., 2003; currently research associate, Center for Magnetic Resonance, Florence, Italy), Dr. Xiangbo Zhao (Ph.D., 2006; currently postdoc Magliozzo lab, Brooklyn College), Chiu Hong Lee (M.A. student, currently CUNY College Lab Tech) and Jun He (M.A., 2003; currently CUNY College Lab Tech); undergraduate students: 18 mentored at Brooklyn College for Honors Research, or NIH-MARC, or LSAMP programs; high school students: 10 mentored for Intel and Siemens Scholarship research. 2006
