Shaneen M. Singh

Education:

Postdoctoral Research Associate, Weill Medical College - 2004 (Computational Biology)

Postdoctoral Research Associate, University of Medicine and Dentistry - 2001 (Biochemistry & Molecular Biology)

Ph.D., Thapar Institute of Engineering and Technology (Patiala, India) - 1998 (Biotechnology)

M.S. , Punjabi University (Patiala, India) - 1993 (Biotechnology)

B.S., Punjabi University (Patiala, India) - 1991 (Life Sciences)

Areas of Expertise:

The long-term research goal of the lab is to apply computer modeling to gain insight into cellular signal transduction pathways, specifically to provide deeper insight into both the normal and aberrant subcellular targeting of domains contained in proteins which are part of macromolecular complexes and function in various biological processes.

Books and Publications

Chavadi. S. S., Edupuganti, U.R., Verrgnolle, O., Fatima, I., Singh, S.M., Soll, C.E., and Quadri, L.E.N. (2011) . "Inactivation of tesA reduces cell-wall lipid production and increases drug susceptibility in mycobacteria." The Journal of Biological Chemistry. 286, 24616-25 [PMID: 21592957] (Books and Publications: Book) 2011

Wywial, E. and S.M. Singh. "Identification and Structural Characterization of FYVE Domain-containing Proteins of Arabidopsis thaliana." BMC Plant Biology 10: 157. (Books and Publications: Peer Reviewed Article) 2010

Wywial, E., J. Haven, S.R. Casjens, Y.A. Hernandez, S. Singh, E.F. Mongodin, C.M. Fraser-Liggett, B.J. Luft, S.E. Schutzer and W.G. Qiu. "Fast, Adaptive Evolution at a Bacterial Host-resistance Locus: The PFam54 Gene Array in Borrelia burgdorferi." Gene 445: 26-37. (Books and Publications: Peer Reviewed Article) 2009

Wywial, E., V.N. Dongre and S. Singh. "Proteomic Tools for the Analysis of Cytoskeleton Proteins." Cytoskeleton Methods and Protocols, 2nd ed., Methods in Molecular Biology Series. Ed. Ray H. Gavin. (Books and Publications: Chapter) 2009

Garcia, M., M. Abbasi, S. Singh and Q. He. "Role of Drosophila Gene dunc-115 in Nervous System." Invertebrate Neuroscience 7: 119-28. (Books and Publications: Peer Reviewed Article) 2007

Kachroo, D., S. Singh and V. Ramamurthy. "Modulation of Unsaturated Fatty Acids Content in the Algae Spirulina platensis and Chlorella minutissima in Response to Herbicide SAN 9785." Electronic Journal of Biotechnology 9. ISSN: 0717-3458. (Books and Publications: Peer Reviewed Article) 2006

Mulgrew-Nesbitt, A., K. Diraviyam, J. Wang, S. Singh, P. Murray, Z. Li, L. Rogers, N. Mirkovic and D. Murray. "The Role of Electrostatics in Protein-membrane Interactions. Lipid-protein Interactions." Biochimica et Biophysica Acta 176: 1812-26. (Books and Publications: Peer Reviewed Article) 2006

Hisert, K.B., M. MacCoss, M.U. Shiloh, K.H. Darwin, S. Singh, R.A. Jones, S. Ehrt, Z. Zhang, B.L. Gaffney, S. Gandotra, D.W. Holden, D. Murray and C. Nathan. "An EAL Domain Protein of Salmonella Controls Bacterial Survival in Mice, Anti-oxidant Defense, and Killing of Macrophages: Role of cyclic-diGMP." Molecular Microbiology 56: 1234-45. (Books and Publications: Peer Reviewed Article) 2005

Stahelin, R.V., B. Ananthanarayanan, N.R. Blatner, S. Singh, K.S. Bruzik, D. Murray and W. Cho. "Mechanism of Membrane Binding of the Phospholipase D1 PX Domain." Journal of Biological Chemistry 279: 54819-26. (Books and Publications: Peer Reviewed Article) 2004

Yu, J.W., J.M. Mendrola, A. Audhya, S. Singh, D. Keleti, D.B. DeWald, D. Murray, S.D. Emr and M.A. Lemmon. "Genome-wide Analysis of Membrane Targeting by S. cerevisiae Pleckstrin Homology (PH) Domains." Molecular Cell 13: 677-88. (Books and Publications: Peer Reviewed Article) 2004

Singh, S. and D. Murray. "Molecular Modeling of the Membrane Targeting of Phospholipase C Pleckstrin Homology Domains." Protein Science 12: 1934-53. (Books and Publications: Peer Reviewed Article) 2003

Singh, S., N. Awasthi, C. Egwuagu and B.J. Wagner. "Immunoproteasome Expression in a Non-immune Tissue, the Ocular Lens." Archives of Biochemistry and Biophysics 405: 147-53. (Books and Publications: Peer Reviewed Article) 2002

Awards, Honors and Fellowships

NSF grant, for "Arabidopsis 2010." $740,100; 2006-10. (Grants and Fellowships) 2006

Conferences, Seminars and Symposiums

Heavner, Mary E., Gwenelle Guenguen, Shubha Govind and Shaneen M. Singh. "Dynamin-like proteins from the VLPs of the parasitoid wasp Leptopilina heterotoma: Unexpected domain architectures." 7th Summer Meeting, New York Structural Biology Discussion Group. New York, Aug. 9. (Conferences, Seminars and Symposiums: Conference Presentation) 2012

Itrat, F. and Singh S. Genome Computational Analysis of GRAM domains in the model plant Arabidopsis thaliana. RECOMB 2012, the 16th Annual International Conference on Research in Computational Molecular Biology, Barcelona, Spain. (Conferences, Seminars and Symposiums: Conference Presentation) 2011

Qi, Y. and Singh, S. Computational Modeling of Cyclooxygenase in Drosophila. 51st Annual Meeting of the American Society for Cell Biology. 2011, Denver, Colorado. (Conferences, Seminars and Symposiums: Conference Presentation) 2011

Weich, E., H. Yu, H. Cheng and S.M. Singh. "Computational Analysis of Sinorhizobium meliloti ExoR Protein." 50th Annual Meeting of the American Society for Cell Biology. Philadelphia, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2010

Wywial, E., N. Dongre and S.M. Singh. "Functional Characerization of Tubby Domains of Arabidopsis thaliana Using Computational Methods." 54th Annual Meeting of the Biophysical Society. San Francisco, February. (Conferences, Seminars and Symposiums: Conference Presentation) 2010

Brasse, D., R. Abdel-Naby and S. Singh. "Genome-wide Modeling and Analysis of BAR Domains in Arabidopsis thaliana." 53rd Annual Meeting of the Biophysical Society. Boston, February. (Conferences, Seminars and Symposiums: Conference Presentation) 2009

Tsoi, S., S.F.P. Mehr and S. Singh. "Functional Annotation of the MyTH4 and FERM Domains of Tetrahymena thermophila MYO1 Using Bioinformatics Tools." 49th Annual Meeting of the American Society for Cell Biology. San Diego, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2009

Wywial, E. and S. Singh. "Computational Analysis of the Membrane Targeting Domains of the Plant-specific PRAF Proteins." International Conference on Bioinformatics and Computational Biology. Paris, June. (Conferences, Seminars and Symposiums: Conference Presentation) 2009

Dongre, N., D.Y. Brogun and S. Singh. "Functional Characterization of Arabidopsis Tubby Domains Using Computational Methods." 48th Annual Meeting of the American Society for Cell Biology. San Francisco, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2008

Wywial, E. and S. Singh. "Computational Analyses of Phosphoinositide-binding Domains in Arabidopsis thaliana." 48th Annual Meeting of the American Society for Cell Biology. San Francisco, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2008

Dongre, N. and S. Singh. "OCLC, a Novel Organism Centric Literature Curation Database." 47th Annual Meeting of the American Society for Cell Biology. Washington, D.C., December. (Conferences, Seminars and Symposiums: Conference Presentation) 2007

Koors, P., A. Frank, M. Saint-jour and S. Singh. "Genome-wide Analysis of the Pleckstrin Homology Domains of Dictyostelium discodieum." 47th Annual Meeting of the American Society for Cell Biology. Washington, D.C., December. (Conferences, Seminars and Symposiums: Conference Presentation) 2007

Lai, M., T. Shuk, Y. Leon and S. Singh. "Functional Annotation of the MyTH4 Domain Using Bioinformatics Tools." 47th Annual Meeting of the American Society for Cell Biology. Washington, D.C., December. (Conferences, Seminars and Symposiums: Conference Presentation) 2007

Silkov, A., E. Wywial, N. Mirkovic, S. Singh and D. Murray. "Proteomic Analysis of Peripheral Membrane Proteins in the A. thaliana Genome." Plant Genomes, Cold Spring Harbor Laboratories. Cold Spring, N.Y., March. (Conferences, Seminars and Symposiums: Contributed Talk) 2007

Wywial, E. and S. Singh. "Genome-wide Characterization of Two Phosphoinositide Binding Domains of the Arabidopsis thaliana Using High-throughput and Manual Computational Analyses." 47th Annual Meeting of the American Society for Cell Biology. Washington, D.C., December. (Conferences, Seminars and Symposiums: Conference Presentation) 2007

Wywial, E., A. Silkov, D. Murray and S. Singh. "Genome-wide Analysis of the FYVE Domains of Arabidopsis thaliana." 15th Annual International Conference on Intelligent Systems for Molecular Biology & 6th European Conference on Computational Biology. Vienna, July. (Conferences, Seminars and Symposiums: Conference Presentation) 2007

Wywial, E. and S. Singh. "Molecular Modeling Studies of the Pleckstrin Homology Domains of the Gab Family of Proteins." 46th Annual Meeting of the American Society for Cell Biology. San Diego, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2006

Newell, S.A., J. Polle and S. Singh. "Molecular Modeling Studies of Chlamydomonas reinhardtii LHC Proteins." 45th Annual Meeting of the American Society for Cell Biology. San Francisco, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2005

Singh, S.M. and D. Murray. "Role of Non-specific Electrostatics in the Membrane Targeting of Pleckstrin Homology Domains." Biophysical Journal (Annual Meeting Abstracts) 2004: 2906-Pos. (Conferences, Seminars and Symposiums: Conference Presentation) 2004

Singh, S.M., Z.D. Li and D. Murray. "Membrane Targeting of Phospholipase C C2 Domains." Biophysical Journal (Annual Meeting Abstracts) 2003: 270-Pos. (Conferences, Seminars and Symposiums: Conference Presentation) 2003

Singh, S.M. and D. Murray. "Molecular Modeling Studies of Phospholipase C PH Domains." 42nd Annual Meeting of the American Society for Cell Biology. San Francisco, December. (Conferences, Seminars and Symposiums: Conference Presentation) 2002